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rabbit monoclonal anti perilipin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti perilipin
    Rabbit Monoclonal Anti Perilipin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perilipin/Perilipin-1+XP+Rabbit+mAb/pmc13016079-31-0-4
    Average 96 stars, based on 473 article reviews
    rabbit monoclonal anti perilipin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The matricellular protein ADAMTS-like 2 regulates differentiation of skeletal muscle-resident fibro-adipogenic progenitor cells
    Article Snippet: To permeabilize the cell membrane, FAPs were incubated with 0.2 mL methanol (179377-4L-PB, Sigma-Aldrich) for 10 min. Non-specific antibody binding sites were blocked with 0.2 mL of 5% bovine serum albumin in PBS for 1 h. Following blocking, FAPs were rinsed three times with 0.5 mL of PBS for 5 min each. .. FAPs were then incubated with 0.2 mL of primary antibody, diluted in PBS for 2 h. The following primary antibodies were used: PDGFRα (14-1401-82, eBioscience, 1:50), Ki67 (9129, Cell Signaling Technology, 1:200), perilipin (9349T, Cell Signaling Technology, 1:200), αSMA (14-9760-82, eBioscience, 1:200), MyHC (DSHB, #MF 20, 1:50), and collagen type I (ab34710, Abcam, 1:200). .. After incubation, FAPs were rinsed three times with 0.5 mL PBS for 5 min each and incubated with 0.2 mL of the following goat-anti mouse or goat-anti rabbit secondary antibodies diluted at 1:500 in PBST for 1h at RT: Rhodamine Red (111-295-144 or 115-295-146, Jackson Immuno Research) or Alexa Fluor 488 (115-545-146 or 111-545-144, Jackson Immuno Research).

    Binding Assay:

    Article Title: The cAMP-phosphodiesterase PDE4B2 controls peroxisome proliferator-activated receptor γ (PPARγ) expression and the initiation of adipogenesis in 3T3-L1 cells
    Article Snippet: Similar amounts of detergent extracts prepared from 3T3-L1 cells or wildtype (WT) and knockout (KO) mouse tissue were separated by SDS/PAGE and subsequently subjected to immunoblotting (IB) using antibodies against PDE4B (#ab170939, RRID:AB_2927553, Abcam, Waltham MA), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #G9545, RRID:AB_796208, Millipore Sigma, St. Louis, MO), or α-Tubulin (#T9026, RRID:AB_477593, Millipore Sigma, St. Louis, MO). .. Antibodies against hormone-sensitive lipase (HSL; #CS18381, RRID:AB_2798800), perilipin (#CS9349, RRID:AB_10829911), fatty acid binding protein 4 (FABP4; #CS2120, RRID:AB_2102466), CCAAT/enhancer binding protein α (C/EBPα; #CS8178, RRID:AB_11178517), C/EBPβ (#CS3087, RRID:AB_2078052), C/EBPδ (#CS2318, RRID:AB_2078194), peroxisome proliferator-activated receptor γ (PPARγ, #CS2435, RRID:AB_2166051), and Phospho-(Ser/Thr) protein kinase A (PKA)-Substrate Antibody (#CS9621, RRID:AB_330304) were from Cell Signaling Technologies (Danvers, MA). .. Immunoblot band intensities were quantified using ImageJ software (RRID:SCR_003070, NIH, Bethesda, MD, USA), and changes in protein expression were calculated as the relative change in the respective treatment group compared to the “no-siRNA group” after normalizing target gene expression against the expression of loading controls (e.g., GAPDH or α-Tubulin).

    Western Blot:

    Article Title: PIK3CA gain-of-function mutation in Schwann cells leads to severe neuropathy and aerobic glycolysis through a non-cell autonomous effect
    Article Snippet: .. List of primary antibodies used in the study Antibody target Catalog # Supplier Working Dilution Application bêta-actine 3700 Cell Signaling Technology 1:10000 WB MBP 40390 abcam 1:1000/1:100 WB/IF MPZ 57518 Cell Signaling 1:1000/1:100 WB/IF GFP ab13970 Abcam 1:1000/1:100 WB/IF Glucagon ab10988 Abcam 1:100 IF Glut1 NB110-39113 Novus Biologicals 1:100 IF Glut2 NBP2-22218 Novus Biologicals 1:100 IF Glut3 NBP1-89762 Novus Biologicals 1:100 IF Glut4 654 Abcam 1:1000 IF IGFBP1 af1240 Novus Biologicals 1:5000 WB Insulin 20056 Immunostar 1:600 IHC Insulin Receptor Beta 23413 Cell Signaling Technology 1:1000 WB Leptin ab16227 Abcam 1:100 IF p110α 4249 Cell Signaling Technology 1:1000 WB p-AKT (Ser473) 4060 Cell Signaling Technology 1:1000 WB p-AKT (Thr308) 2965 Cell Signaling Technology 1:100 IF Perilipin-1 9349 Cell Signaling Technology 1:1000 WB p-S6RP 5364 Cell Signaling Technology 1:1000/1:100 WB/IF α-Tubulin t5168 Merck 1:10000 WB S100 beta ab4066 Abcam 1:1000/1:100 WB/IF Cre-recombinase 15036 Cell Signaling Technology 1:100 IF Perilipin-1 NBP3-13615 Biotechne 1:100 IF Supplementary Table 1: List of primary antibodies used in the study Table S2. ..

    Immunohistochemistry:

    Article Title: PIK3CA gain-of-function mutation in Schwann cells leads to severe neuropathy and aerobic glycolysis through a non-cell autonomous effect
    Article Snippet: .. List of primary antibodies used in the study Antibody target Catalog # Supplier Working Dilution Application bêta-actine 3700 Cell Signaling Technology 1:10000 WB MBP 40390 abcam 1:1000/1:100 WB/IF MPZ 57518 Cell Signaling 1:1000/1:100 WB/IF GFP ab13970 Abcam 1:1000/1:100 WB/IF Glucagon ab10988 Abcam 1:100 IF Glut1 NB110-39113 Novus Biologicals 1:100 IF Glut2 NBP2-22218 Novus Biologicals 1:100 IF Glut3 NBP1-89762 Novus Biologicals 1:100 IF Glut4 654 Abcam 1:1000 IF IGFBP1 af1240 Novus Biologicals 1:5000 WB Insulin 20056 Immunostar 1:600 IHC Insulin Receptor Beta 23413 Cell Signaling Technology 1:1000 WB Leptin ab16227 Abcam 1:100 IF p110α 4249 Cell Signaling Technology 1:1000 WB p-AKT (Ser473) 4060 Cell Signaling Technology 1:1000 WB p-AKT (Thr308) 2965 Cell Signaling Technology 1:100 IF Perilipin-1 9349 Cell Signaling Technology 1:1000 WB p-S6RP 5364 Cell Signaling Technology 1:1000/1:100 WB/IF α-Tubulin t5168 Merck 1:10000 WB S100 beta ab4066 Abcam 1:1000/1:100 WB/IF Cre-recombinase 15036 Cell Signaling Technology 1:100 IF Perilipin-1 NBP3-13615 Biotechne 1:100 IF Supplementary Table 1: List of primary antibodies used in the study Table S2. ..

    Immunopeptidomics:

    Article Title: Differential pathology and susceptibility to MBNL loss across muscles in myotonic dystrophy mouse models
    Article Snippet: .. Antibodies included the following: Laminin (MilliporeSigma, L9393, 1:1,000), eMHC/ MYH3 (Developmental Studies Hybridoma Bank [DSHB], F1.652-s, 1:40), MHC-I (DSHB, BA-F8-c, 1:400), MHC-IIA (DSHB, SC-71-c, 1:300), MHC-IIB (DSHB, BF-F3-c, 1:200), MHC-IIX (DSHB, 6H1-s, 1:20), DAPI (MilliporeSigma, D9542, 1:25,000), and Perilipin (Cell Signaling Technology, 9349S, 1:1000). ..

    Staining:

    Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis
    Article Snippet: .. Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100). .. Fluorescence-conjugated secondary antibodies (Jackson ImmunoResearch, 1:200) were used in immunofluorescence procedures to detect fluorescent signals.



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